Composite

Part:BBa_K3733043:Design

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-10-01)


HepT suicide system working at low temperatures


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 82


Design Notes

None


Source

Used a strong RBS to produce high levels of expression of the HepT protein. Design Considerations:This is a composite part including the RNA thermometer(hot-inhibit) and the HepT toxin.


References